Thermo
Scientific
v
Water Jacketed CO2 Incubator
Thermo Scientific
Preface
Warranty Notes
Information You Should Know Before Requesting Warranty Service
•
Locate the model and serial numbers. A serial tag is located on the unit itself.
• For equipment service or maintenance, or with technical or
special application inquiries, contact Technical
Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local
distributor.
Repairs NOT Covered Under Warranty
• Calibration of control parameters. Nominal calibrations
are performed at the factory; typically ±1°C for
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
a billable service at your location. Calibration after a warranty repair is covered under the warranty.
• Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
• Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1) electrical
service connection
2) tubing connections
3) gas regulators
4) gas tanks
5) unit leveling
6) room ventilation
7) adverse ambient
temperature fluctuations
8) any repair external to the unit
• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
• Repairs to parts or systems resulting from unauthorized unit modifications.
• Any labor costs other than that specified during the parts and labor warranty period, which may
include additional warranty on CO
2
sensors, blower motors, water jackets, etc.
Water Jacketed CO
2
Incubator vi
Thermo Scientific
Table of Contents
Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Name and Description of Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2
Control Panel Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Installing the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Stacking the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Installing the Access Port Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Installing Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Installing HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Installing Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Leveling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Connecting Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . . . . .1-9
Filling Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Filling Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Connecting CO
2
or N
2
Gas Supply . . . . . . . . . . . . . . . . . . . . . . . .1-12
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-13
Setting the Operating Temperature . . . . . . . . . . . . . . . . . . . . . . . .1-14
Setting the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . .1-14
Setting CO
2
Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-15
Setting O
2
Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-16
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Calibrating the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Temperature Stabilization Periods . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Calibrating T/C CO
2
System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
T/C CO
2
Sensor Stabilization Periods . . . . . . . . . . . . . . . . . . . . . . .2-3
Calibrating
Infra-Red CO
2
System . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
IR CO
2
Sensor Stabilization Times . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Calibrating the O
2
System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
O
2
Calibration at 20.7% . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
O
2
Offset Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Calibrating Relative Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6
Relative Humidity Stabilization Times . . . . . . . . . . . . . . . . . . . . . .2-6
Section 1
Section 2
vii
Water Jacketed CO
2
Incubator
Thermo Scientific
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Turning the Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . .3-1
New HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Setting the REPLACE HEPA Filter Reminder . . . . . . . . . . . . . . . . .3-2
Setting an Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Setting Low Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Setting High Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Enabling Temp Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . .3-4
Setting Low CO
2
Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Setting High CO
2
Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Enabling CO
2
Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . .3-5
Setting New Zero # for T/C CO
2
Sensors . . . . . . . . . . . . . . . . . . . . .3-5
Setting New Span # for T/C CO
2
Sensors . . . . . . . . . . . . . . . . . . . . .3-6
Setting a Low RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7
Enabling RH Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . .3-7
Setting a Low O
2
Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7
Setting
a High O
2
Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-8
Enabling O
2
Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . . .3-8
Enabling Temp/RH to be Displayed . . . . . . . . . . . . . . . . . . . . . . . . .3-9
Enabling CO
2
/O
2
to be Displayed . . . . . . . . . . . . . . . . . . . . . . . . . . .3-9
Selecting a Primary Tank (Gas Guard) . . . . . . . . . . . . . . . . . . . . . . .3-10
Disabling the Gas Guard System . . . . . . . . . . . . . . . . . . . . . . . . .3-10
Setting a RS485 Address (1535 Only) . . . . . . . . . . . . . . . . . . . . . . .3-10
Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Inner Door Open Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
REPL O2 SNSR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
O2 SNSR ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
REPL IR SNSR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
CO2 SNSR ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
IR AUTOZ ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Cleaning the Interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Cleaning Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Cleaning Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Reversing the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Reversing Hinges for Exterior Door . . . . . . . . . . . . . . . . . . . . . . . .5-4
Replacing Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
HEPA Filter Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Replacing Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Replacing Access Port Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Draining Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Table of Contents
Section 3
Section 4
Section 5