The Impact of Intensive Fish Farming on Pond Sediment Microbiome and Antibiotic Resistance Gene Composition

Conflict of Interest:
The authors declare that the research was conducted in the
absence of any commercial or financial relationships that could be construed as a
potential conflict of interest.
Copyright © 2021
Lastauskien˙e, Valskys, Stankeviˇci¯ut˙e, Kalcien˙e, G˙egžna,
Kavoli¯unas, Ružauskas and Armalyt˙e. This is an open-access article distributed
under the terms of the Creative Commons Attribution License (CC BY). The use,
distribution or reproduction in other forums is permitted, provided the original
author(s) and the copyright owner(s) are credited and that the original publication
in this journal is cited, in accordance with accepted academic practice. No use,
distribution or reproduction is permitted which does not comply with these terms.
Frontiers in Veterinary Science | www.frontiersin.org
12
May 2021 | Volume 8 | Article 673756

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Document Outline

• The Impact of Intensive Fish Farming on Pond Sediment Microbiome and Antibiotic Resistance Gene Composition
  • Introduction
  • Materials and Methods
    • Sediment Sample Collection
    • Determination of Heavy Metal Concentration
    • Detection of Antibiotic Residues by HPLC-MS
    • Sediment Toxicity Bioassay
    • DNA Extraction
    • Microbiome Analysis
      • Sequencing
      • 16S rRNA Data Analysis
    • Antibiotic Resistance Gene Detection
  • Results
    • Heavy Metal Content Analysis
    • Determination of the Antibiotic Residues in Sediments
    • Sediment Toxicity
    • Microbiome Analysis
    • Antibiotic Resistance Gene Detection
  • Discussion
  • Data Availability Statement
  • Author Contributions
  • Funding
  • Acknowledgments
  • Supplementary Material
  • References