89
Deem, S.L. 2001. “Jaguar (Panthera onca) health evaluation results.” Report submitted to
the Wildlife Conservation Society and the
Simon Bolivar Zoological Park, Costa Rica.
Fransen, D.R. 1972-1973. Feline infectious peritonitis in an infant jaguar. In: Proc. Am. Assoc.
Zoo Vet. Houston, TX, 1972 and Columbus, OH, 1973. p 261-264.
Hoogesteijn R., and E. Mondolfi. 1992. The Jaguar. Caracas: Armitano Publishers.
Patton, S., A. Rabinowitz, S. Randolph, and S. Strawbridge. 1986. A coprological survey of
parasites of wild neotropical felidae. J. Parasit. 72: 517-520
90
General Bibliography
Almeida, A. de. 1974. Jaguar hunting in the Mato Grosso and Bolivia. Long Beach:
Woodbine-Safari Press. 1990.
Brown, D. E., and C. A. Lopez Gonzales. 2001. Borderland Jaguars. Salt Lake City: University
of Utah Press.
Carmony, N. B.1989. Onza !: The hunt for a legendary cat. Silver City: High-Lonesome Books.
1995.
Eisenberg J. F. 1989. Mammals of the neotropics: The northern neotropics. Volume 1. Chicago:
The University of Chicago Press.
Guggisberg, C. 1975. Wild Cats of the World. New York: Taplinger Press.
Hall, E. R., and W. W. Dalquest. 1963. The mammals of Veracruz. University of Kansas:
Museum of Natural History Publication. p 165-362.
Hoogesteijn, R., and E. Mondolfi. 1992. The Jaguar. Caracas: Armitano Publishers.
Nowell, K., and P. Jackson. 1996. IUCN Wild Cats: Status Survey and Conservation Action
Plan. Gland: IUCN.
Perry, R. 1970. The World of the Jaguar. Newton Abbot: David and Charles Ltd.
Quigley, H. B. 1987. Ecology and Conservation of the Jaguar in the Pantanal Region, Mato
Grosso do Sul, Brazil. Moscow: Ph.D. dissertation. University of Idaho, Moscow.
Rabinowitz, A. 1986. Jaguar. New York: Arbor House.
Watt, E. M. 1989. Jaguar Women. Toronto: Key Porter Books.
(For more resources on the jaguar we recommend An Extensive Bibliography on the Jaguar
published by E. Lee Fitzhugh which includes over 866 citations. For a disk copy of the entire
bibliography please e-mail Robert Wiese at bob@fortworthzoo.org and indicate MS WORD or
WordPerfect format).
91
Acknowledgement and thanks go out to all those who contributed to this work. The Jaguar
Species Survival Plan is indebted to them for written chapters, financial and development
support, and time spent on review, editing and comments.
Christopher Law, Editor, Philadelphia Zoo; telephone: (215) 243-5316,
e-mail: chris_law@prodigy.net
William K. Baker, Jr., Little Rock Zoo; telephone: (501) 666-2406 x102,
e-mail: puma_cat@hotmail.com
Sharon Deem DVM, National Zoological Park; telephone: (202) 673-7980,
e-mail: deems@nzp.si.edu
Amy Hunt, MS, Fort Worth Zoo; telephone: (817) 759-7145; ahunt@fortworthzoo.org
Linda Munson DVM, University of California; telephone: (530) 754-7567,
e-mail: lmunson@ucdavis.edu
Stacey Johnson, Fort Worth Zoo; telephone (817) 759-7190; e-mail: sjohnson@fortworthzoo.org
Rebecca Spindler, Ph.D., National Zoological Park; telephone: (540) 635-6594
e-mail: rspindler@crc.si.edu
Ann Ward, MS, Fort Worth Zoo; telephone: (817) 759-7141; e-mail: award@fortworthzoo.org
Dominic Calderisi, Lincoln Park Zoo
Stephanie Crowson, Fort Worth Zoo
Brent Day, Little Rock Zoo
Marie Greene, Little Rock Zoo
Stacey Johnson, Fort Worth Zoo
Gray Lang, Dallas World Aquarium
Gwen Lovett, Palm Beach Zoo
Keith Lovett, Palm Beach Zoo
Stephanie Lubianski, Houston Zoo
Danny Morris, Omaha's Henry Doorly Zoo
Jim Schnormeier, Sacramento Zoo
Alan Shoemaker, Columbia, SC
Rebecca Spindler, National Zoo
Becky Volk, Fort Worth Zoo
Chris Waldron, Philadelphia Zoo
John Ward, Fort Worth Zoo
Diana Weinhardt, Houston Zoo
Robert Wiese, Fort Worth Zoo
Patti Hainley, Panthera Research
Debbie Thompson, Little Rock Zoo
Formatted
March 2003
1
A Field Manual for the Collection, Storage, and Transportation of Biomaterials for
Genetic Studies on Jaguars (
Panthera onca)
George Amato, Ph.D.
Cathi Lehn, Ph.D.
Wildlife Conservation Society
TABLE OF CONTENTS
I.
Introduction
II.
Biomaterial
Sampling for Genetic Studies
III.
Literature Cited
IV.
Appendices
The following guidelines are intended for field biologists with previous wildlife
experience whom are working in association with the Wildlife Conservation Society
(WCS) Jaguar Conservation Program (JCP). They have been developed by the WCS
Science Resource Center (SRC) and personnel working with JCP. Training sessions and
consultation on biomaterial sampling, genetic analyses, and specific research issues
related to genetic investigations of jaguars can be arranged through consultation with
the SRC and JCP staff (see Appendix I for contact information).
The main objectives for the conservation genetics component of the JCP are: 1) to
provide standardized methods for the collection of biomaterials from jaguars; 2) to
provide assistance in the experimental design of genetic research projects; 3) to
recommend the application of genetic techniques to questions of concern in the
management and conservation of the jaguar; 4) to assist in the interpretation of genetic
results; and; 5) to provide recommendations, based on genetic findings, for the long-
term management and conservation of the jaguar.
I. INTRODUCTION
1
The application of molecular techniques to the management of endangered animals has
become an invaluable tool to the conservation biologist (Moritz 1994; Avise 1996; Smith
and Wayne, 1996; Ashley 1999). Blood and tissue samples (liver, heart, kidney, spleen,
muscle and brain) are the preferred biomaterial for genetic studies. Deoxyribonucleic
acid (DNA) extractions from tissue recover high molecular weight DNA and produce a
high yield from a small portion of the sample. Although blood is a good source of high
quality DNA, the ratio of mitochondrial to nuclear DNA in blood samples is extremely