Forma Series II 3110 Water-Jacketed co2 Incubators User Manual

IR AUTOZ ERR
I/R Units
Water Jacketed CO
2
Incubator        4-3
Thermo Scientific
Section 4
Alarms
If the cables or connectors between the main microprocessor board and the
CO
2
sensor, or between the CO
2
board and the sensor head on I/R CO
2
units become loose or disconnected, the CO2 SNSR ERR alarm will
occur.
On I/R incubators, the red light on the I/R module (see Figure 5-4 for the
module circuit board location) is lit continuously if communication is lost
between the CO
2
board and the sensor head. The CO
2
display is locked at
00.0 without injection. Turning the incubator off and on will not clear the
alarm. Only proper connections of all the components corrects the alarm.
On incubators equipped with I/R CO
2
control, calibration is performed
automatically using an Auto Zero system. Auto Zero occurs once every 24
hours. If, during the Auto Zero cycle, a CO
2
correction of more than
0.45% is detected, the IR AUTOZ ERR alarm will occur.
Possible problems which will cause this alarm are:
•
Auto Zero pump, orifice, filter or tubing will not allow air to the
sensor. 
Possibilities are: 
~ Defective or electrically disconnected air pump
~ Kinked auto zero vinyl tubing
~ Disconnected tubing between air pump and sensor
~ Plugged filter or orifice on auto zero assembly
~ Defective auto zero circuit 
•
Cabinet temperature has been increased significantly from a previous
setpoint. (For example, the unit was calibrated and operating at 30°C
and the setpoint is increased to 50°C) In this instance, calibrating the
CO
2
will correct the alarm.
•
There is high background CO
2
in the laboratory. This could be from
leaks in the tank, regulator or vinyl CO
2
tubing.
•
High CO
2
sensor calibration drift occurred. This requires replacement
of the sensor.
CO2 SNSR ERR

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Action 
Daily Weekly Monthly  6 
Months 
Yearly 2 
years 
Check CO
2
 tank levels. 
 
 
 
 
 
 
Inspect door latch, hinges and door gasket seal. 
 
 
 
 
 
 
Check water level in the humidity pan, ½” from top. See “Filling the 
Humidity Pan”. 
 
 
 
 
 
 
Verify and document CO
2
, O
2
, humidity and temperature calibration, as 
applicable. See Calibration section 
 
 
 
 
 
 
Perform a complete decontamination procedure. Wipe down interior, shelves 
and side ducts with disinfectant. Change or clean blower wheel and scroll. 
Clean top duct. Clean humidity pan. Rinse everything well with sterile 
distilled water. See “Cleaning the Interior, “Cleaning the Humidity Pan”. 
Between experiments 
More frequent decontamination may be required, 
depending on use  and environmental conditions 
Change HEPA and gas filters, if applicable (or as needed). See “Installing Air 
Sample Filter”, “Installing HEPA Filter”. 
 
 
 
 
 
 
Replenish rust inhibitor in water jacket. See “Adding/Replenishing Rust 
Inhibitor”. 
 
 
 
 
 
 
Replace AquaTec water cleaning cell, if applicable. 
 
 
 
 
 
 
 
 
PREVENTIVE MAINTENANCE 
Incubators 
 
Your equipment has been thoroughly tested and calibrated before shipment. Regular preventive maintenance is important to keep your unit 
functioning properly. The operator should perform routine cleaning and maintenance on a regular basis. For maximum performance and 
efficiency, it is recommended the unit be checked and calibrated periodically by a qualified service technician. 
 
The following is a condensed list of preventive maintenance requirements. See the specified section of the operating manual for further 
details. 
 
We have qualified service technicians, using NIST traceable instruments, available in many areas. For more information on Preventive 
Maintenance or Extended Warranties, please contact us at the number listed below. 
 
Cleaning and calibration adjustment intervals are dependent upon use, environmental conditions and accuracy required. 
 
Tips for all incubators: 
 
 
Do NOT use bleach or any disinfectant that has high chloros 
 
Use sterile, distilled or demineralized water. 
 
Avoid spraying cleaner on the CO
2
 sensor. 
 
Do not use powdered gloves for tissue cultures. 
 
 

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Water Jacketed CO
2
Incubator        5-1
Thermo Scientific
Section 5  
Routine Maintenance
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users must check with the
manufacturer that the proposed method will not damage the equipment. 
▲
Warning If the units have been in service, disconnect the power cord
connector before disinfecting. 
▲
Use an appropriate disinfectant.  All articles and surfaces must be
thoroughly cleaned, rinsed and rough-dried.
Warning Alcohol, even a 70% solution, is volatile and flammable Use it
only in a well ventilated area that is free from open flame. If any
component is cleaned with alcohol, do not expose the component to open
flame or other possible hazard.  Allow the alcohol to fully dry before
turning power on. 
▲
Warning Do not spray the T/C sensor with flammable solutions.  The
internal temperature of the CO
2
sensor is approximately 150°C when the
unit is in operation. Allow sufficient time for the sensor to cool before
cleaning. 
▲
Warning Do not use strong alkaline or caustic agents.  Stainless steel is
corrosion resistant, not corrosion proof. Do not use solutions of sodium
hypochlorite (bleach), as they may also cause pitting and rusting. 
▲
1. Turn the incubator off and disconnect the plug from the power source.
2. Remove the shelves, access port
filter and side duct sheets.
Remove the temperature sensors
and the air sample filter tubing
from the back of the blower
scroll. If unit is equipped with
the optional RH sensor,
unfasten it from the clip on the
top duct. See Figure 5-1.
Cleaning the Interior
Figure 5-1. Sensor and Filter Locations

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