Dna fingerprinting Gel Staining and Analysis Student Instructions Gel Staining and Analysis Step 1

DNA Fingerprinting Gel Staining and Analysis Student Instructions

Gel Staining and Analysis Step 1

• When electrophoresis is completed, turn off the power supply.

• Remove the lid from the box.

• Lift the lid to remove it from the box.

Gel Staining and Analysis Step 2

• To stain the gel to make the DNA band visible, remove the gel tray from the electrophoresis unit and place the tray into a plastic container.

• Slide the gel off the tray by pushing on one end of it.

Gel Staining and Analysis Step 3

• Add the Carolina Blu DNA Stain, making sure the gel is completely immersed. Do not pour the stain directly on the gel.

• Stain the gel for 15 minutes. Agitate gently, if possible.

Gel Staining and Analysis Step 4

• Pour the stain back into the bottle.

• Cover the gel with distilled water to destain.

• Agitate gently, if possible, for 30 to 40 minutes, changing the water every 10 minutes.

Gel Staining and Analysis Step 5

• The gel can be displayed to the class by sliding it onto a piece of plexiglass and placing it on a white sheet of paper or on a white-light viewing box.

Gel Staining and Analysis Step 6

• Ideal gel

• Stained with ethidium bromide and viewed under UV light source