Disease name

OIE Reference Laboratory Reports

Activities in 2010

Name of disease (or topic) for which you are a designated OIE Reference Laboratory:	Equine infectious anemia
Address of laboratory	National Veterinary Services Laboratories USDA, APHIS, VS P.O. Box 844, 1920 Dayton Avenue Ames, IA 50010, USA
Tel.:	(+1-515) 337.75.51
Fax:	(+1-515) 337.73.48
e-mail address:	[e-mail]
website:	http://www.aphis.usda.gov/animal_health/lab_info_services
Name of Head of Laboratory (Responsible Official):	Dr Elizabeth A. Lautner
Name of OIE Reference Expert:	Dr Eileen N. Ostlund
Name of writer of this report (if different from above):

Part I: Summary of general activities related to the disease

1. Test(s) in use/or available for the specified disease/topic at your laboratory

Test	For	Specificity	Total
ELISA	Antigen	Type	621
AGID	Antibody	Type	11,159
WB	Antibody	Type	8

2. Production and distribution of diagnostic reagents

Type of reagent	Amount supplied nationally (including for own use)	Amount supplied to other countries
Weak positive AGID reference serum	671 vials of 2 ml	19 vials of 2 ml
Strong positive AGID reference serum	67 vials of 2 ml	40 vials of 2 ml
Negative AGID reference serum	34 vials of 2 ml	14 vials of 2 ml
EIA proficiency test panels (20 samples/panel)	500 sets of 20 vials, 0.6 ml per vial	2 sets of 20 vials, 0.6 ml per vial

The OIE Members receiving EIA reagents included Argentina, Brazil, Costa Rica, Netherlands, Saudi Arabia, and United Kingdom.

Part II: Activities specifically related to the mandate
of OIE Reference Laboratories

3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines

EIA serum panels are prepared and used in annual certification of approximately 500 laboratories in the United States of America. These panels are available to other countries upon request. The OIE Reference Laboratory participated in an international quality assurance EIA antibody panel test supplied by the Australian National Quality Assurance Program (ANQAP) and worked with ANQAP regarding discrepant test results.

4. Preparation and supply of international reference standards for diagnostic tests or vaccines

Reference Standards provided are listed in Reagents section (#2). No OIE recognized reference standards were produced.

5. Research and development of new procedures for diagnosis and control

The Reference Laboratory provided consultation and participated in pre-license evaluation of a new EIA ELISA test kit for a commercial company. In the United States, EIA antibody detection ELISA and AGID diagnostic kits undergo final evaluation for licensure by the United States Department of Agriculture’s Center for Veterinary Biologics. Licensed diagnostic kits are available commercially from several manufacturers. Proficiency panels for EIA are provided to the Center for Veterinary Biologics, USDA, for use in diagnostic test evaluations. No EIA vaccine is approved for use in the United States of America.

6. Collection, analysis and dissemination of epizootiological data relevant to international disease control

The number and location (state) of EIA positive equids is compiled each year by the United States Department of Agriculture, Animal and Plant Health Inspection Services, National Animal Health Surveillance System.

This data is available at: http://www.aphis.usda.gov/vs/nahss/equine/eia/eia_distribution_maps.htm.

7. Provision of consultant expertise to OIE or to OIE Members

Laboratory personnel respond to technical queries from OIE Members as requested. Summary data on EIA proficiency panels is provided to OIE Members participating in proficiency panel testing.

8. Provision of scientific and technical training to personnel from other OIE Members

No requests for scientific and technical training on EIA were received from OIE members.

9. Provision of diagnostic testing facilities to other OIE Members

Diagnostic assistance was provided to France following detection of an EIA positive horse on standard pre-import serology submission. The laboratory confirmed reporting of the positive results, by France, to OIE. Samples from numerous OIE member countries were tested for EIA prior to animal movement to the United States. The World Equestrian Games (WEG) was held in the United States during 2010. The Reference Laboratory provided pretesting of over 400 samples from horses in OIE Member countries to assure they would successfully be allowed to enter the United States for the WEG. The Reference Laboratory also provided the EIA testing for all WEG horses entering the country by plane at the various US import centers, including a temporary import center constructed for the WEG competition horses.

10. Organisation of international scientific meetings on behalf of OIE or other international bodies

None.

11. Participation in international scientific collaborative studies

None.

12. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)

None.

13. Inscription of diagnostic kits on the OIE Register

i) Did you participate in expert panels for the validation of candidate kits for inscription on the OIE Register? If yes, for which kits?

This activity was not conducted in 2010.

ii) Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for which kits?

This activity was not conducted in 2010.

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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010